Zootermopsis angusticollis- Dampwood termite

December 1, 2022

Sample information
Picture
Photos by: Chase Hartzell, Heather Ahlstrom
Location
Collection date 09/06/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
  1. Zootermopsis angusticollis- Dampwood termite- Under rotting log under the brush along path (same as millipede)

Description: Red head with tan waxy body

Body length: 0.9cm Found by Carly, Chase, and Alex Hartzell

UTM Coordinates: 12T 0492214 4797321

Arthropod: +? Wolbachia: +
Putative identification Arthropoda Hexapoda Insecta Isoptera Archotermopsidae Zootermopsis Zootermopsis angusticollis

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

DNA Annotated protocol in Wolbachia project Lab2: DNA Extraction Edward’s Buffer

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Retested  and re-PCR and ran on gel again and sent to the Wolbachia Project lab. Had the sequence done and confirmed the match on Blast.
Wolbachia 16S sequence
>Wol15 GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGAATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Zootermopsis angusticollis was found to be postive for Wolbachia.
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