Wolbachia #1: Ant

March 31, 2023

Sample information
Picture
Photos by: Julia B.
Location
Collection date 03/25/2023
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations
  • entire body was intact
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Used a 2% Gel.


Well 1: Ladder (100bp)

Well 2:  (+) DNA Control

Well 3: (-) Wolbachia

Well 4: (+) Wolbachia

Well 5:  arthropod #1 (ant)

Well 6: arthropod #2 (ant)

Well 7: (-) DNA Control/Water

Well 8: Nothing

Well 9:Nothing

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

As the other arthropod’s band did not show up, it is possible that errors were made that would skew results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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