Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations |
|
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Used a 2% Gel.
Well 2: (+) DNA Control Well 3: (-) Wolbachia Well 4: (+) Wolbachia Well 5: arthropod #1 (ant) Well 6: arthropod #2 (ant) Well 7: (-) DNA Control/Water Well 8: Nothing Well 9:Nothing |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | As the other arthropod’s band did not show up, it is possible that errors were made that would skew results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman