|Location||No map location data available.|
|Captive / Cultivated?||Wild-caught|
|Group||Woburn Memorial High School|
Normal sized bumble bee, loud buzzing. Did not fight back when trying to collect.
|Putative identification||Hexapoda Insecta Hymenoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||MiniOne|
Followed the Wolbachia project protocol.
2 percent gel.
Gel electrophoresis gel identification:
Well 1: DNA Ladder
Well 2: Nothing
Well 3: Bee Sample
Well 4: Blue Mosquito Sample
Well 5: Wolbachia Control (Positive)
Well 6: Wolbachia Control (Negative)
Well 7: DNA Control (Positive)
Well 8: Water
|Explanation of confidence level||
The bands in my gel were very clear, and there was no signs of any cross contamination in my experiment. All of my controls were correct, so my confidence in the validity of the experiment is strong.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Hymenoptera was found to be negative for Wolbachia.|