Bumble Bee- Wolbachia Negative

March 31, 2023

Sample information
Picture
Photos by: Ava C
Location No map location data available.
Collection date 09/14/2022
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

Normal sized bumble bee, loud buzzing. Did not fight back when trying to collect.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed the Wolbachia project protocol.

 

2 percent gel.

Gel electrophoresis gel identification:

Well 1: DNA Ladder

Well 2: Nothing

Well 3: Bee Sample

Well 4: Blue Mosquito Sample

Well 5: Wolbachia Control (Positive)

Well 6: Wolbachia Control (Negative)

Well 7: DNA Control (Positive)

Well 8: Water

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The bands in my gel were very clear, and there was no signs of any cross contamination in my experiment. All of my controls were correct, so my confidence in the validity of the experiment is strong.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
Report Inappropriate Post