Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | Normal sized bumble bee, loud buzzing. Did not fight back when trying to collect. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Followed the Wolbachia project protocol.
2 percent gel. Gel electrophoresis gel identification: Well 1: DNA Ladder Well 2: Nothing Well 3: Bee Sample Well 4: Blue Mosquito Sample Well 5: Wolbachia Control (Positive) Well 6: Wolbachia Control (Negative) Well 7: DNA Control (Positive) Well 8: Water |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The bands in my gel were very clear, and there was no signs of any cross contamination in my experiment. All of my controls were correct, so my confidence in the validity of the experiment is strong. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman