Sample Information: Spider arthropod

Sample information
Picture	Photos by: Marakii W.
Location
Collection date	04/13/2023
Captive / Cultivated?	Wild-caught
Group	Arroyo High School
Observations
Putative identification	Arthropoda Chelicerata
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	body without wings
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level	I am not confident because we aren’t Wolbachia positive and if they did have Wolbachia it would have shown since from our gel we can see that the DNA ladder did in fact work as intended.
Wolbachia 16S sequence
Arthropod COI sequence	The Chelicerata was found to be negative for Wolbachia. BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Chelicerata was found to be negative for Wolbachia.

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