Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | We aren’t confident because we aren’t Wolbachia positive and if they did have Wolbachia it would have shown since from the gel we can see that the DNA ladder in fact worked as intended. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
The insects was found to be negative for Wolbachia.
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Insecta was found to be negative for Wolbachia. |
Water Boatman