Pardosa milvina (F-3)

November 20, 2023

Sample information
Picture
Photos by: Charlie L.
Location
Collection date 09/15/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Eight skinny black joined legs. Dark grey & mustard yellow head and
cephalothorax with an off-white abdomen
Putative identification Arthropoda Arachnida

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The used pippet tip went into liquid but the used tip was not used, just submerged in the liquid. It was used twice with non-DNA sample liquids.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No mistakes were made that could possibly contaminate DNA.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Arachnida was found to be negative for Wolbachia.
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