Sample information

I found this mosquito near an alleyway between Palm Beach Christian Academy and Rinker School of Business in the afternoon, about 2:00 pm, in 87 degrees F. I attached a picture of when the mosquito was contained in a petri dish.

Methods

DNA extraction: made sure samples are crushed well and entire body used

Used 2% agarose gel

Used 4 uL of 6X loading dye

Gel electrophoresis lanes:

1 – 10 uL 1 Kb Plus ladder

2 – (no sample)

3 – 20 uL of arthropod #1 (mosquito)

4 – 20 uL of arthropod #2 (ant)

5 – 20 uL of positive arthropod control

6 – 20 uL of negative arthropod control

7 – 20 uL of positive DNA control

8 – 20 uL of water

Analysis: controls worked and extraction done properly because bands for arthropod gene are seen and all three control bands were as expected. Lane 8 is negative control with just water therefore no bands.

Results

All controls were as shown as expected results. No conflicts encountered during lab procedure. The size of arthropod CO1 gene is calculated to be 764 bp and Wolbachia band of 409 bp was not detected in the mosquito.