Mosquito “Culicidae”

Sample information

Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University

I found this mosquito near an alleyway between Palm Beach Christian Academy and Rinker School of Business in the afternoon, about 2:00 pm, in 87 degrees F. I attached a picture of when the mosquito was contained in a petri dish.

Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae


Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: made sure samples are crushed well and entire body used

Used 2% agarose gel

Used 4 uL of 6X loading dye

Gel electrophoresis lanes:

1 – 10 uL 1 Kb Plus ladder

2 – (no sample)

3 – 20 uL of arthropod #1 (mosquito)

4 – 20 uL of arthropod #2 (ant)

5 – 20 uL of positive arthropod control

6 – 20 uL of negative arthropod control

7 – 20 uL of positive DNA control

8 – 20 uL of water

Analysis: controls worked and extraction done properly because bands for arthropod gene are seen and all three control bands were as expected. Lane 8 is negative control with just water therefore no bands.


Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls were as shown as expected results. No conflicts encountered during lab procedure. The size of arthropod CO1 gene is calculated to be 764 bp and Wolbachia band of 409 bp was not detected in the mosquito.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culicidae was found to be negative for Wolbachia.
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