Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Caught in the pine mulch at the base of a tree by the Student Union at the Armstrong GSU campus. Caught at around 10:00am in the shade using the tray method. It was very fast moving. |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My positive was a faint positive, though my professor said she would call it a positive. My gel was also missing the DNA ladder. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be postive for Wolbachia. |
Water Boatman