MACACDF23#22

November 7, 2023

Sample information
Picture
Photos by: Patricia O.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in the pine mulch at the base of a tree by the Student Union at the Armstrong GSU campus. Caught at around 10:00am in the shade using the tray method. It was very fast moving.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My positive was a faint positive, though my professor said she would call it a positive. My gel was also missing the DNA ladder.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
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