Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | 0945 AM in grassy field along the parking lot by Science Drive. Implemented the sweep method for collection. Weather conditions were partly cloudy and humid. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | I believe all steps of the experiment were performed correctly and with care, however, I was not the only person to handle the sample which implies there could have been some error that I cannot personally account for. The results indicate that my controls were successful and that my sample is positive for the Wolbachia gene. My partner did have an inconclusive result so I think it would be best to consider the possibility of error in all our group’s samples. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be postive for Wolbachia. |
Water Boatman