|Captive / Cultivated?||Wild-caught|
|Group||Georgia Southern University|
0945 AM in grassy field along the parking lot by Science Drive. Implemented the sweep method for collection. Weather conditions were partly cloudy and humid.
|Putative identification||Arthropoda Hexapoda Insecta Hemiptera|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
DNA extraction kit of in-house reagents was used
|Explanation of confidence level||
I believe all steps of the experiment were performed correctly and with care, however, I was not the only person to handle the sample which implies there could have been some error that I cannot personally account for. The results indicate that my controls were successful and that my sample is positive for the Wolbachia gene. My partner did have an inconclusive result so I think it would be best to consider the possibility of error in all our group’s samples.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Hemiptera was found to be postive for Wolbachia.|