LOGACEF23-JT

November 12, 2023

Sample information
Picture
Photos by: Jillian T.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

0945 AM in grassy field along the parking lot by Science Drive. Implemented the sweep method for collection. Weather conditions were partly cloudy and humid.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I believe all steps of the experiment were performed correctly and with care, however, I was not the only person to handle the sample which implies there could have been some error that I cannot personally account for. The results indicate that my controls were successful and that my sample is positive for the Wolbachia gene. My partner did have an inconclusive result so I think it would be best to consider the possibility of error in all our group’s samples.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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