GREACB23-13 Hemiptera

November 13, 2023

Sample information
Picture
Photos by: Mar H
Location No map location data available.
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Date: 8/21/2023

Time Collected: 1:17 PM

Location: Left field near Aquaponics Center of Armstrong Campus

Environment: Short and tall grass

Collection Method: Sweep Net & Bug Jar

Weather: Hot and sunny with partial clouds and light rain
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Medium confidence due to positive control bands and sample bands matching, only medium due to negative control and no template control not showing up
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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