Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Fast moving and flees from light. Attempts to wriggle free and bite after caught. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Gonads dissected |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | Our confidence level is low due to the failure of the controls. The – Wolbachia control did show a positive band which likely means that there was cross contamination between the samples. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)