Eastern Red Centipede

April 26, 2024

Sample information
Picture
Photos by: John O.
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Fast moving and flees from light. Attempts to wriggle free and bite after caught.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Our confidence level is low due to the failure of the controls. The – Wolbachia control did show a positive band which likely means that there was cross contamination between the samples.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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