Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Has wings but does not fly away. Can fly in short bursts. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Gonads dissected |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | The confidence level is low because the – Wolbachia control showed a band which means that there was likely contamination at some point in the process. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Water Boatman