Biting Midge

April 26, 2024

Sample information
Picture
Photos by: John O.
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

Has wings but does not fly away. Can fly in short bursts.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The confidence level is low because the – Wolbachia control showed a band which means that there was likely contamination at some point in the process.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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