Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | brown, long, thin, many legs, many small segments |
| Putative identification | Arthropoda Myriapoda Diplopoda Julida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Other |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | |
| Gel images |
|
| Protocol notes | DNA extraction location: 3rd segment of organism |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | When we ran the gels, the controls created the correct bands, but there were some smudged lines in the negative control well. Also, we might not have isolated the right segment of the millipede. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Julida was found to be negative for Wolbachia. |
Water Boatman