Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Gymnasium Immensee |
| Observations | |
| Putative identification | Arthropoda Chelicerata Arachnida |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We had control-DNA extracts from positive and negative samples. These two samples were used
|
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arachnida was found to be negative for Wolbachia. |
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata