Sweat Bee

December 7, 2023

Sample information
Picture
Photos by: Addyson T
Location
Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

The Sweat Bee was located outside, sitting on the ledge of a picnic table. I used my collection tube to slowly trap the bug inside. After I caught the Bee I placed it Pitri Disk, to further investigate it.
Putative identification Arthropoda

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

We froze our samples halfway through extraction. During extraction I dropped one of the samples on the floor. The sample did not seem to spill out.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

I am not super confident in my results because, some of the bands and controls didn’t make sense or did not add up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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