Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/12/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Well 1: DNA ladder well 2: empty well 3: 1-AR spider sample well 4: positive arthropod control well 5: negative arthropod control well 6: positive DNA control well 7: water negative control well 8: empty well 9: empty |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The Wallachia controls did not work this there was nothing to compare the possible arthropod contamination to. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata