1- AR

March 6, 2024

Sample information
Picture
Photos by: Alice R
Location
Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Well 1: DNA ladder

well 2: empty

well 3: 1-AR spider sample

well 4: positive arthropod control

well 5: negative arthropod control

well 6: positive DNA control

well 7: water negative control

well 8: empty

well 9: empty

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The Wallachia controls did not work this there was nothing to compare the possible arthropod contamination to.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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