Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/05/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | Yellow spider with a darker shade triangle on its back, 8 legs, seems to be around 8mm in length with its legs being larger than its body.
|
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Cheiracanthiidae Cheiracanthium |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Due to the fact that the positive control arthropod in well 4 showed no band at 438 bp indicating Wolbachia prescience the rest of the experiment is invalidated. It remains inconclusive if the arthropod being observed in well 2 is infected with wolbachia because there is no control to reference it to. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Cheiracanthium was found to be negative for Wolbachia. |
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata