Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/29/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | right back leg lost, only has 5 legs |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes |
|
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My control worked; the DNA tested positive for arthropod DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
In the Gel image, my data is in lanes 6 and 7. Lane 6 is my control for testing for Arthropod DNA. Lane 7 is my test for Wolbachia.
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)