Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Found on a magnolia tree leave at eye-level, slightly damp from rain, humid outside. Collected by tearing leaf off of tree and shaking into net. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | When measuring concentration, I calculated a value of 2.08. When looking over gel electrophoresis results, all controls were accurately displayed. Other students in my class with Coleoptera insects also had positive results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coccinellidae was found to be postive for Wolbachia. |
Water Boatman