DEMSp18-3

April 10, 2024

Sample information
Picture
Photos by: Dillon W.
Location
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected the insect in the outside campus grass field adjusted to the science center parking lot.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident in the results provided because my quality of DNA was good (1.7) and the gel electrophoresis worked as expected with the positive and negative controls showing that my sample was positive for Wolbachia and insect DNA
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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