Rhagium inquisitor AB1

Sample information
Picture	Photos by: Ann B.
Location
Collection date	03/14/2024
Captive / Cultivated?	Wild-caught
Group	KantiWil
Observations	It was found in a forest under the bark of deadwood, where it was wet.
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Cerambycidae
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	The confidence level is high because there was a cytochromase band but no wolbachia band
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA Download AB1 CTTTACTACTTTCWAGAGGATTAGTTGAAAGAG BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Cerambycidae was found to be negative for Wolbachia.

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