Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | The test object was found in the forest under a rotten tree trunk on humid dirt. It was alone and crawling quickly below a leave. |
| Putative identification | Arthropoda Myriapoda Chilopoda Lithobiomorpha Lithobiidae Lithobius Lithobius forficatus |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Although two bands are clearly visible only one of them counts (700 band for cytochrome oxidase). The other one that is lower than 430). This band was produced by a primer-dimear formation because of no sequence results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TAWTTCGATTAGAATTAAGCCAACCAGGAAGATTAATTGGAGACGACCAAATATATWATGYTGTAGTTWYTGCCCAYGSWTTTATWATAATCTTTTTTATAGTTATACCAATTATAATTGGGGGATTTGGAAATTGATTAGTTCCMCTAATWYTATGYKYWGYWGAYATAGCCTTTCCCCGGTTAAATAATATAAGATTTTGACTTCTGCCCCCCTCATTAACTTTACTATTATGCTCTGCTGCAGTAGAGAGAGGGGCAGGAACAGGATGAACTGTATACCCCCCCCTATCTGCTAATATTTCCCATAGCGGKGCATCAGTAGACATAACCATTTTCTCTCTTCATCTTGCCGGAGCCTCTTCAATTTTAGGGGCCATTAATTTTATCTCTACMATTATCAATATACGAACAAGAGGAATAACTTTTGAACGAGTACCTCTCTTTGTATGATCCGTAAAAATTACCGTTATCCTCCTTCTTTTATCTCTCCCGGTACTGGCTGGCGCTATCACCATACTACTTACTGACCGAAACCTAAACACGAGATTCTTTGACCCCACAGGAGGAGGAGATCCTATTTTATACCAGCACTTATTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Lithobius forficatus was found to be negative for Wolbachia. |
Water Boatman