Camponotus pennsylvanicus

May 1, 2024

Sample information
Picture
Photos by: Malak A.
Location
Collection date
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2023 Students
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My confidence is high because when analyzing the gel electrophoresis, there was a 438 bp band present on the gel, similar to the band present for +Wolbachia.
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA   
Summary The Camponotus pennsylvanicus was found to be postive for Wolbachia.
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