Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Cockroach caught from a tiny hole on CAMS campus in cement. Lured out using puffcorn pieces. |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea Ectobiidae |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | Both gels were run for around 30 minutes using orange g loading dye, then Fast Blast with 100x for 3 minutes. Then the gels were rinsed under warm water for 10 seconds and destained with warm water for 5 minutes off the rocker and 5 minutes on a slow rocker. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Our gel provided very faint bands, so it hard to tell whether the pcr for the Wolbachia provided enough sample to visualize the band. This data is somewhat reliable because the positive Wolbachia and arthropod controls both contained a Wolbachia band in the Wolbachia pcr gel along with all the arthropod samples containing the same size bad in the arthropod pcr gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Ectobiidae was found to be negative for Wolbachia. |
Water Boatman