GRP3 – Cockroach

May 24, 2024

Sample information
Picture
Photos by: Benjamin N
Location
Collection date 05/14/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Cockroach caught from a tiny hole on CAMS campus in cement. Lured out using puffcorn pieces.
Putative identification Arthropoda Hexapoda Insecta Blattodea Ectobiidae

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Both gels were run for around 30 minutes using orange g loading dye, then Fast Blast with 100x for 3 minutes. Then the gels were rinsed under warm water for 10 seconds and destained with warm water for 5 minutes off the rocker and 5 minutes on a slow rocker.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Our gel provided very faint bands, so it hard to tell whether the pcr for the Wolbachia provided enough sample to visualize the band.  This data is somewhat reliable because the positive Wolbachia and arthropod controls both contained a Wolbachia band in the Wolbachia pcr gel along with all the arthropod samples containing the same size bad in the arthropod pcr gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ectobiidae was found to be negative for Wolbachia.
Report Inappropriate Post