Sample information

Wildcaught. Caught near Pogonomyrmex barbatus, black colored, crawling around the soil near school campus.

Methods

For Wolbachia Gel:

*Wells starting near ruler

Lane 1: HindIII ladder w/ UView

Lane 2: H20 w/ UView

Lane 3: Positive DNA control w/ UView

Lane 4: Negative Arthropod control w/ UView

Lane 5: Positive Arthropod control w/ UView

Lane 6: Arthropod #1 (Pogonomyrmex barbatus)

Lane 7: Arthropod #2 (Monomorium minimum)

Lane 8: Blank

For Arthropod Gel:

*Wells starting near ruler

Lane 1: Blank

Lane 2: HindIII ladder w/ UView

Lane 3: H20 w/ UView

Lane 4: Positive DNA control w/ UView

Lane 5: Negative Arthropod control w/ UView

Lane 6: Positive Arthropod control w/ UView

Lane 7: Arthropod #1 (Pogonomyrmex barbatus)

Lane 8: Arthropod #2 (Monomorium minimum)

Setting: 100V constant, 45 minutes

*Using Separate Power Boxes

Results

Expected bands for both gels appeared on the gel. Both arthropod and DNA control had worked out, shown by the bands that are on the gels. As controls had work, nothing that is seemingly out of the ordinary had occurred, so certain that this arthropod test negative for Wolbachia. However, not high confidence as we believe that we made minor human error throughout entire process, unknown whether or not these had an impact on running gel.