Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/21/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Spider with 8, clear-like legs. Dark brown bulb with cream-colored spots. Size of a grain of rice. Caught in the bush of the lowest parking lot, with forceps and petri dishes. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Oecobiidae Oecobius Oecobius navus |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | 100 volt, 60+ minutes |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | There were no bands visible after running the gel and staining it overnight, so we are very confident the results are inconclusive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman