GRP4 Camponotus

May 25, 2024

Sample information
Picture
Photos by: Katherine L., Parishi J., Ritu G., Emma L.
Location
Collection date 05/13/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Found on a tree at CAMS, active, yellow stripes on abdomen, brown color, shaded area
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Ran the gel for 60 minutes at 105 volts.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our confidence level was determined by the success in our DNA Ladder and controls. The Arthropod CO1 and Wolbachia-specific 16S rRNA genes were present in both the (+) Arthropod control and (+) DNA control in our Arthropod PCR samples. Additionally, the (-) Arthropod control contained only the CO1 band and no Wolbachia band in our Arthropod PCR side, and there were no bands or smudges in our (-) water controls. This indicates that the extraction and PCR processes of extracting the genes were successful.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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