Sample information

small head and a broad, flattened body, reddish-brown, large compound eyes, two ocelli, and long, flexible antennae. The arthropod was caught outside of a manhole at night in an urban area. The season was fall and the temperature was about 76 degrees.

Methods

DNA Extraction

1. The DNA extraction went well for the most part. The only issues of note are that we added about 50ul of extra Buffer to B-2, B-3, and B-5. We also spilled a little bit of B-5.

PCR”:

1. There was one notable mistake during the PCR, we forgot to switch pipette tips between samples possibly contaminating them.

Gel electrophoresis, Arthropod CO1

1. The ladder was marked on all Gels
2. Samples B-1 through B-6 were positive for arthropod DNA 
3. The positive and negative controls were also marked positive and water was marked negative

Gel electrophoresis, Wolbachia

1. B-1 through B-5 were negative and B-6 was positive
2. The controls worked with both negative and water controls marked negative and the positive control marked positive
3. There were no notable mistakes or errors

Results

There was a point in the experiment where pipette tips were not switched between samples although I don’t believe there was enough contamination to ruin the samples as there was one that had Wolbachia and others that didn’t. The controls were also correct improving our confidence in the gels.