B-3 Cockroach

October 30, 2024

Sample information
Picture
Photos by: Lucas S
Location
Collection date 09/18/2024
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

small head and a broad, flattened body, reddish-brown, large compound eyes, two ocelli, and long, flexible antennae
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer Other
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was a point in the experiment where pipette tips were not switched between samples although I don’t believe there was enough contamination to ruin the samples as there was one that had Wolbachia and others that didn’t.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be negative for Wolbachia.
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