Sample information |
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Picture |
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Location | |
Collection date | 09/24/2024 |
Captive / Cultivated? | Wild-caught |
Group | Edmund Burke School |
Observations |
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Putative identification | Arthropoda Hexapoda Insecta Orthoptera Rhaphidophoridae Tachycines Tachycines asynamorus |
Methods |
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Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes |
DNA Extraction
PCR”:
Gel electrophoresis, Arthropod CO1
Gel electrophoresis, Wolbachia
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Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | There was a moment when we forgot to switch pipettes between samples but it doesn’t seem to have affected the experiment. The controls worked as planned further proving the validity of our gels. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Tachycines asynamorus was found to be negative for Wolbachia. |