B-4 Emma Field Cricket

Sample information

Picture
Photos by: Lucas S
Location
Collection date
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

The Teleogryllus Emma Cricket has a dark brown to black body with a shiny shell. It has long, thin antennae, strong back legs for jumping, and flat wings with visible patterns. The eyes are prominent, and its hind legs have small spikes.

 

Putative identification

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer Other
DNA stain Other
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was a moment when we forgot to switch pipettes between samples but it doesn’t seem to have affected the experiment

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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