Roly Poly

March 31, 2025

Sample information
Picture
Photos by: https://drive.google.com/file/d/1CC4QDrBE-zxItYuZlMhW2FqrN5fywnp9/view?usp=sharing
Location
Collection date 09/12/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Small arthropod identified as an “Armadillidium vulgare,” a.k.a. a “Roly-Poly.” The subject’s habitat was a moist dirt patch in a grass yard, although it was photographed in a plastic tube. The “Armadillidium vulgare” has a brown shell back,14 small white legs, and two antennas.
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

I dropped the reproductive parts in the wrong tube before PCR and had to extract it out which may have lead to faulty data

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

There was no DNA present in the electrophoresis tank indicating an error in either DNA extraction or PCR
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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