Unknown Moth

March 29, 2021

Sample information
Picture
Photos by: Isabella R.
Location
Collection date 10/03/2020
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

found in suburban front yard on a blade of grass beneath a Bradford pear tree; fluttered around low to the ground

~1.5cm, looks like a small moth, two antennae, two mouth parts, cream-colored
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be postive for Wolbachia.
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