Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 12/11/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | FernLeaf Community Charter School |
| Observations | We found a spider (xysticus) |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | We started with scooping the guts out for the DNA. We than extracted the DNA using buffers, and put that DNA into the electrophoresis system for 30 minutes. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We ran it through the same process as the Stinkbug (previously posted). The negatives came back negative, and the positives came back positive, the tested water also come back negative. However the spider came back negative. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Araneae was found to be negative for Wolbachia. |
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