Sample information
Picture	Photos by: Mia E.
Location
Collection date	03/18/2021
Captive / Cultivated?	Wild-caught
Group	KantiWil
Observations	found under a tree bark, weather was rather cold with snow
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Silphidae Phosphuga Phosphuga atrata
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Abdomen
Single or Duplex PCR
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Phosphuga atrata was found to be negative for Wolbachia.