Phosphuga atrata (ME2)

April 29, 2021

Sample information
Picture
Photos by: Mia E.
Location
Collection date 03/18/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

found under a tree bark, weather was rather cold with snow
Putative identification Arthropoda Hexapoda Insecta Coleoptera Silphidae Phosphuga Phosphuga atrata

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phosphuga atrata was found to be negative for Wolbachia.
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