NV 78 Mosquito

April 18, 2021

Sample information
Picture
Location No map location data available.
Collection date
Captive / Cultivated? Captive / Cultivated
Group Neuqua Valley High School
Observations

It was caught onto a spider web but it had wings so therefore it was a mosquito.
Putative identification Arthropoda Myriapoda Chilopoda

Methods
Extraction kit Puregene (Qiagen)
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer LB
DNA stain GelGreen
Gel images
Protocol notes

With the results, I think it turned out to be Wolbachia negative.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I don’t know if I looked at the results completely correct which is why my confidence level is very low for this.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chilopoda was found to be negative for Wolbachia.
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