Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/31/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | It is brown and black. It has seven pairs of legs and two pairs of antennae. Its body is segmented. |
| Putative identification | Arthropoda Malacostraca |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Other |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My pellet was visible and black and big. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Arthropod control -A did not seem to work. -A and +A control in wolbachia gel did not seem to work. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Malacostraca was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid