|Captive / Cultivated?||Wild-caught|
We noticed these beetles that would come out every night around 8pm and bother us. They were loud and would run into us. Not very intelligent, they would routinely get stuck on their backs and would not be able to do anything until they fell off the surface they were on. There were so many of them! We thought they had something to do with the grape plant next to our fence, grape beetles look very similar but have spots. These have no spots. We identified them as lawn grubs, and these are May/June beetles.
|Putative identification||Hexapoda Insecta Coleoptera Scarabaeidae Phyllophaga|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Partial abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system|
DNA extraction: Followed protocol, removed exoskeleton and extracted goop from inside the bug. I tried to get as much of the white goop as possible, and as little of the exoskeleton.
|Explanation of confidence level|
|Wolbachia 16S sequence||
|Arthropod COI sequence||