May beetle

Sample information

Picture
Photos by: Athena L.
Location
Collection date 04/08/2021
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

We noticed these beetles that would come out every night around 8pm and bother us. They were loud and would run into us. Not very intelligent, they would routinely get stuck on their backs and would not be able to do anything until they fell off the surface they were on. There were so many of them! We thought they had something to do with the grape plant next to our fence, grape beetles look very similar but have spots. These have no spots. We identified them as lawn grubs, and these are May/June beetles.

Putative identification Hexapoda Insecta Coleoptera Scarabaeidae Phyllophaga

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

DNA extraction: Followed protocol, removed exoskeleton and extracted goop from inside the bug. I tried to get as much of the white goop as possible, and as little of the exoskeleton.

 

Results

Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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