Beetle

May 5, 2021

Sample information
Picture
Photos by: Nathan S.
Location
Collection date 03/30/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

A small black bug with 6 legs and no visible wings. It has two parts to it, and it has 2 antennas and ridges on the shell.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Partial abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My pellet was small cloudy and green.

 

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

DNA Ladder in arthropod gel didn’t work. Positive and negative controls didn’t work. W gel control didn’t work. Maybe problems with DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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