|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
A small black bug with 6 legs and no visible wings. It has two parts to it, and it has 2 antennas and ridges on the shell.
|Putative identification||Hexapoda Insecta Coleoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Partial abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
My pellet was small cloudy and green.
|Explanation of confidence level||
DNA Ladder in arthropod gel didn’t work. Positive and negative controls didn’t work. W gel control didn’t work. Maybe problems with DNA extraction.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Coleoptera was found to be negative for Wolbachia.|