Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | A small black bug with 6 legs and no visible wings. It has two parts to it, and it has 2 antennas and ridges on the shell. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My pellet was small cloudy and green.
|
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | DNA Ladder in arthropod gel didn’t work. Positive and negative controls didn’t work. W gel control didn’t work. Maybe problems with DNA extraction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman