Earwig

May 5, 2021

Sample information
Picture
Location
Collection date 04/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Has intentas, is dark brown with hints of light brown, has ridges, has six legs, and has a tail that is split into two
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Pellet was visible. It was a brownish color. DNA ladder for arthropod gel is faint, no bands in water for arthropod, in positive arthropod there is a band, in negative arthropod there isn’t a band. In arthropod 02, 04, 11, 12, and 13 there are bands. In arthropod 01, 03, 04, 05, 06, 07, 08, and 09 there isn’t a band. In wolbachia  02, 04, 05, 11, and 13 there are bands. In wolbachia 01, 03, 06, 07, 08, 09, and 12 there aren’t bands.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

DNA ladder in arthropod gel didn’t work. Positive and negative controls did not work. Wolbachia gel control did not work. Maybe problems with DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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