|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
Has intentas, is dark brown with hints of light brown, has ridges, has six legs, and has a tail that is split into two
|Putative identification||Hexapoda Insecta Dermaptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
Pellet was visible. It was a brownish color. DNA ladder for arthropod gel is faint, no bands in water for arthropod, in positive arthropod there is a band, in negative arthropod there isn’t a band. In arthropod 02, 04, 11, 12, and 13 there are bands. In arthropod 01, 03, 04, 05, 06, 07, 08, and 09 there isn’t a band. In wolbachia 02, 04, 05, 11, and 13 there are bands. In wolbachia 01, 03, 06, 07, 08, 09, and 12 there aren’t bands.
|Explanation of confidence level||
DNA ladder in arthropod gel didn’t work. Positive and negative controls did not work. Wolbachia gel control did not work. Maybe problems with DNA extraction.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Dermaptera was found to be negative for Wolbachia.|