Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/01/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Has intentas, is dark brown with hints of light brown, has ridges, has six legs, and has a tail that is split into two |
| Putative identification | Arthropoda Hexapoda Insecta Dermaptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Pellet was visible. It was a brownish color. DNA ladder for arthropod gel is faint, no bands in water for arthropod, in positive arthropod there is a band, in negative arthropod there isn’t a band. In arthropod 02, 04, 11, 12, and 13 there are bands. In arthropod 01, 03, 04, 05, 06, 07, 08, and 09 there isn’t a band. In wolbachia 02, 04, 05, 11, and 13 there are bands. In wolbachia 01, 03, 06, 07, 08, 09, and 12 there aren’t bands. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | DNA ladder in arthropod gel didn’t work. Positive and negative controls did not work. Wolbachia gel control did not work. Maybe problems with DNA extraction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Dermaptera was found to be negative for Wolbachia. |
Water Boatman