Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/07/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Norwich Free Academy |
| Observations | The spider has a black body with yellow legs. The size is less than half an inch. It was found by a tree in a park. It was walking around in the dirt. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | The spider DNA is in the fifth well. The spider is very small so using the whole body was fine. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | My control didn’t show. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Araneae was found to be negative for Wolbachia. |
Water Boatman