|Captive / Cultivated?||Wild-caught|
|Group||Norwich Free Academy|
The spider has a black body with yellow legs. The size is less than half an inch. It was found by a tree in a park. It was walking around in the dirt.
|Putative identification||Chelicerata Arachnida Araneae|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||Fast Blast|
The spider DNA is in the fifth well. The spider is very small so using the whole body was fine.
|Explanation of confidence level||
My control didn’t show.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Araneae was found to be negative for Wolbachia.|