Spider

July 16, 2021

Sample information
Picture
Photos by: Aubrey R.
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

The spider has a black body with yellow legs. The size is less than half an inch. It was found by a tree in a park. It was walking around in the dirt.
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

The spider DNA is in the fifth well. The spider is very small so using the whole body was fine.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My control didn’t show.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneae was found to be negative for Wolbachia.
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