Spider

July 16, 2021

Sample information
Picture
Location
Collection date 07/06/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

This tiny spider has eight legs and lives on the bark of trees.
Putative identification Arthropoda Chelicerata Arachnida Araneae Araneidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

I used the entire bug. It is located in the second well.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

This bug was not positive with Wolbachia. My confidence is low because the positive control didn’t show up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneidae was found to be negative for Wolbachia.
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