Harvestmen

July 16, 2021

Sample information
Picture
Photos by: Fiona L.
Location
Collection date 07/06/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

I found it in a field of grass while standing up. It was also near a stream, it was also 88 degrees, we were near a shaded tree when we caught it. The grass wasn’t tall, the legs are long and it goes skinnier as it goes down. There are long lines on the body, the skin texture is different than others. The skin texture is is black on top and on the bottom whiter than the top.
Putative identification Arthropoda Hexapoda Insecta Orthoptera Phalangopsidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

I used the whole bug and put it in a tube and mashed it up.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We messed up in the beginning by adding the SS with the positive and later on added the SS in the second to last well. The bands also didn’t show up that much and didn’t show that good of a results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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