Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/06/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Norwich Free Academy |
| Observations | Segmented, oval body, long antennae, 6 legs, brown in color, collected in shaded area in leaf litter. |
| Putative identification | Arthropoda Hexapoda Insecta Blattodea Cryptocercidae Cryptocercus |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | Used abdomen of wood roach. In 3rd well. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | Band was blurry, positive control N/A, slightly lower than SS |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Cryptocercus was found to be postive for Wolbachia. |
Water Boatman