Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/25/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | St. John High School USD 350 |
| Observations | The scorpions were collected on and around a backyard wall using a black light after dark. The stingers were removed for safety. |
| Putative identification | Arthropoda Chelicerata Arachnida Scorpiones |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | The scorpions were stored in a freezer for about 2 weeks until extraction. DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took one day to complete. PCR: Mini One Taq polymerase was used. Gel Electrophoresis: Mini PCR was used. All of the lanes worked well except for Lane 2. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I am confident Lane 3, 4, and 5 are positive because they are double banded. There was something wrong with Lane 2 which may be due to an error in DNA extraction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | The Scorpiones was found to be postive for Wolbachia. |
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