Arizona Bark Scorpions

July 13, 2021

Sample information
Picture
Location
Collection date 05/25/2021
Captive / Cultivated? Wild-caught
Group St. John High School USD 350
Observations

The scorpions were collected on and around a backyard wall using a black light after dark. The stingers were removed for safety.
Putative identification Arthropoda Chelicerata Arachnida Scorpiones

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The scorpions were stored in a freezer for about 2 weeks until extraction.

DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took one day to complete.

PCR: Mini One Taq polymerase was used.

Gel Electrophoresis: Mini PCR was used. All of the lanes worked well except for Lane 2.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident Lane 3, 4, and 5 are positive because they are double banded. There was something wrong with Lane 2 which may be due to an error in DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Scorpiones was found to be postive for Wolbachia.
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