|Captive / Cultivated?||Wild-caught|
|Group||St. John High School USD 350|
The scorpions were collected on and around a backyard wall using a black light after dark. The stingers were removed for safety.
|Putative identification||Arthropoda Chelicerata Arachnida Scorpiones|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniPCR|
The scorpions were stored in a freezer for about 2 weeks until extraction.
DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took one day to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: Mini PCR was used. All of the lanes worked well except for Lane 2.
|Explanation of confidence level||
I am confident Lane 3, 4, and 5 are positive because they are double banded. There was something wrong with Lane 2 which may be due to an error in DNA extraction.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Scorpiones was found to be postive for Wolbachia.|