Fly larvea

July 16, 2021

Sample information
Picture
Photos by: Nora G
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

Found in leaf litter near river.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

This bug is in the third well. I used the entire bug because it was small then a grain of rice.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

This bug was not positive with Wolbachia. My confidence is low because the positive control didn’t show up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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