|Captive / Cultivated?||Wild-caught|
|Group||Norwich Free Academy|
Found in leaf litter near river.
|Putative identification||Hexapoda Insecta|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||Fast Blast|
This bug is in the third well. I used the entire bug because it was small then a grain of rice.
|Explanation of confidence level||
This bug was not positive with Wolbachia. My confidence is low because the positive control didn’t show up.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Insecta was found to be negative for Wolbachia.|