Mosquito Wolbachia Sample

July 21, 2021

Sample information
Picture
Photos by: Angelina R.
Location
Collection date 06/11/2020
Captive / Cultivated? Wild-caught
Group South Plainfield High School
Observations

The mosquito is a female Culex.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There is a clear band indicating the presence of wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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