Sample information
Picture	Photos by: Angelina R.
Location
Collection date	06/11/2020
Captive / Cultivated?	Wild-caught
Group	South Plainfield High School
Observations	The mosquito is a female Culex.
Putative identification	Arthropoda Hexapoda Insecta Diptera
Methods
Extraction kit	Monarch DNA extraction (NEB)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	There is a clear band indicating the presence of wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Diptera was found to be postive for Wolbachia.