|Captive / Cultivated?||Wild-caught|
|Group||Woburn Memorial High School|
Leaf hopper found in Woburn Memorial highschool grass. It is green and small.
|Putative identification||Hexapoda Insecta Hemiptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||MiniOne|
Duplex PCR reaction was preformed.
|Explanation of confidence level||
In this lab the question was if our arthropod contained Wolbachia DNA. The purpose of the lab was to isolate DNA from organisms and determine if there is Wolbachia. During this lab we used PCR to amplify arthropod and Wolbachia DNA. The data shows in image 2, that no band is shown in well 2. This well contained my arthropod sample. I can not draw a conclusion from my results because neither the 708 bp band or 438 bp band was present. I think this data may be caused from not properly isolating and purifying the DNA from the sample. In image 1, well 5 PCR #2 the wolbachia double band is present. In well 6, there is only the 708 band which means this sample is wolbachia negative. These samples worked in run 1 (image 1) but were left out in run 2 (image 2). I am unable to draw conclusions for my run 2 results. There is a band present in my DNA control and in my water control. These results make it unable to understand my results because there was contamination in my controls.
|Wolbachia 16S sequence||
Primers provided by Wolbachia projectBLAST at The Wolbachia Project BLAST at NCBI
|Arthropod COI sequence||
Primers provided by Wolbachia ProjectBLAST at The Wolbachia Project BLAST at NCBI