Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | Under a damp log(crawling around on the dirt), tried running away when collecting |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Originally our gel did not set up properly so we had to redo it. And when loading the gels we put it backwards so when we put it in the wash buffer it washed out. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | We loaded our gel backwards so our confidence level was not high at all |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman