PillBug

April 19, 2022

Sample information
Picture
Photos by: T.J. J
Location
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

Under a damp log(crawling around on the dirt), tried running away when collecting
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Originally our gel did not set up properly so we had to redo it. And when loading the gels we put it backwards so when we put it in the wash buffer it washed out.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We loaded our gel backwards so our confidence level was not high at all
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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