Armadillidium vulgare

April 18, 2022

Sample information
Picture
Photos by: Krisha P., Alex P.
Location
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

Under a log in a wet environment with a  plentiful of leaves around it.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

During DNA extraction when the AW 1 and AW 2 buffers were added the solution turned pink

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I think our procedure went well but the pink during DNA extraction may have cause a problem with our results
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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