Drosophila 22

Sample information

Collection date 04/19/2022
Captive / Cultivated? Wild-caught
Group The Summit Country Day School

Wild-caught fruit fly at restaurant.

Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster


Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Standard protocol for DNA extraction and gel electrophoresis to screen for Wolbachia


Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia infected drosphila and Wolbachia uninfected drosophila and water controls all worked correctly.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Drosophila melanogaster was found to be negative for Wolbachia.
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