Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/19/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | The Summit Country Day School |
| Observations | Wild caught drosophila at restaurant. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Standard DNA extraction, duplex PCR and gel electrophoresis per Discover the Microbe Within protocols. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Wolbachia infected drosophila, Wolbachia uninfected drosophila and water controls all worked as expected. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Drosophila melanogaster was found to be negative for Wolbachia. |
Water Boatman